Overview DermaGenius® multiplex real-time PCR assays identify the most prevalent fungal infections in nail, hair and skin samples within 2.5 hours. A semi-automated DNA-extraction is recommended in the procedure to ensure efficiency with limited hands-on time. The procedure from sample to diagnosis can be implemented b
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- RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec. Wide dynamic range from 0.5 pg – 500 pg Multiplex up to 5 targets per assay
- Quantstudio 12K, and Dx system and in-house procedures. Biomek NXP automation workstation. SOP4098, SOP4984 DNA D,C, c Allele-specific PCR / gel electrophoresis PCR using BioRad C1000 Touch Thermal Cycler SOP4097
Nov 29, 2010 · instrument, and (c) the Life Technologies QuantStudio® Dx Real-Time PCR system. C. Measurand: Targets DNA sequences of the toxin A (tcdA), and toxin B (tcdB) genes within the PaLoc of toxigenic strains of Clostridium difficile. D. Type of Test: Nucleic acid amplification test based on multiplex, real-time. E. Applicant: Quidel Corporation
- Get high multiplex performance in an American-made product. Excellent sensitivity with CDC-recommended primers. All enzymes are synthesized in the United States. Fast turn-around times for customization that fits your workflow and format.
Quenchers include 3′ IBFQ alone, double-quenched internal ZEN with 3' IBFQ, or double-quenched internal TAO with 3' IBRQ (Cy5 only). PrimeTime Eco qPCR Probes The PrimeTime Eco qPCR Probe is the ideal scale when you need to perform ~500 reactions for gene expression analysis or genotyping.
- We can multiplex up to 200 samples per run and we use v3 2x300 Illumina MiSeq reagents. Turnaround time: ~4-6 weeks including 16s library construction (DNA is provided) or 3-4 weeks for just the MiSeq run. Other cycle options are available depending on need. Deliverables: Raw data through OTU table data.
Blocks, QuantStudio 3 and 5 systems offer improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.
- 3 / 26 IVD Market In vivo Diagnostics : Analyze the health status inside of the human body. ex) X-ray, CT, MRI In vitro Diagnostics : Analyze the specimens derived from the body such as blood, urine etc.
A slope of –3.322 means that the PCR has an efficiency of 1, or 100%, and the amount of PCR product doubles during each cycle. A slope of less than –3.322 (e.g., –3.8) is indicative of a PCR efficiency <1. Generally, most amplification reactions do not reach 100% efficiency due to experimental limitations.
- 3.1 2.1 1.1 0.1 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Allele 2 Allele 1 Allele Discrimination Plot (SNP Assay: SNP Assay 1) Homozygous 1/1 Heterozygous 1/2 Homozygous 2/2 Undeterminded 4.1 3.6 2.6 0.6 1.6 StepOne and StepOnePlus software automatically determines genotypes and generates an intuitive graphic representation of results in a ...
System gives great flexibility, range of chemistry, and multiplex options • Seamless switching from real-time PCR to digital PCR. Scales to your research • Scales seamlessly from 96 or 384 wells to 3,072 wells • Quick and simple block changes • Automation capable with ample data storage and convenient remote monitoring. The Qua. The ...
- 10X Optimized Buffer(3) - 17.5 mM MgCl 2 - 27.5 mM MgCl 2 - 27.5 mM + detergents; Roche : EHIFI-RO: Expand™ High Fidelity PCR System: No: Clear: Enzyme; 10X Optimized Buffer; 10X Buffer; MgCl 2 Solution; Roche : 2GFMPXKB: KAPA2G Fast Multiplex Mix: No: Clear: PCR master mix; Kapa Biosystems : TAQ-RO: Taq DNA Polymerase, 5 U/μl: No: Clear ...
Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL Description The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use.